Finding the optimal conditions for the production of cellulase enzyme from the fungus Trichoderma viride
Abstract
This research was conducted at Tishreen University during the period 2023-2024, and aimed to determine the optimal conditions for producing the cellulase enzyme from the fungus Trichoderma viride in terms of pH, nutrient media concentration, incubation temperature and time using the response surface technology, by taking advantage of the pods of the Ghaf plant. As a food medium, cellulase enzyme can be used in many industrial applications.
A central composite design was used to verify the effect of four independent variables: pH, concentration of ghaf pods (%), incubation temperature and time on the response factor, which is the effectiveness of cellulase enzyme production. The independent variables were tested at three levels based on the results of the preliminary experiments. Response outcomes were predicted using a second-order polynomial model.
According to this study, the optimal conditions for the production of cellulase by Trichoderma viride were determined, where the pH was = 6, while the concentration of pods was 5.2%, the incubation temperature = 35 °C, and its time = 142 hours, where the expected theoretical values reached (54.8 U.ml). -1), and when these conditions were applied, the enzymatic activity of cellulase reached 53.9 U.ml-1. The convergence of the experimental results with the theoretical values indicated the suitability of the model and the possibility of using the response surface methodology in determining the optimal conditions for enzyme production.
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